|
KMID : 0364219790220020081
|
|
Korean Journal of Zoology
1979 Volume.22 No. 2 p.81 ~ p.93
|
|
|
Effects of Progesterone on the Macromolecular Syntheses in Mouse Preimplantation Embryos in Vitro
|
|
Cho Wan-Kyoo
Kwon Hyuk-Bang
|
|
|
Abstract
|
|
|
|
Metabolic changes of early mouse embryos treated with progesterone were investigated in order to elucidate the mode of action of progesterone on embryogenesis in vitro. The embryos were cultured, and labelled with radioactive precursors of macromolecules for certain periods in the absence or presence of various concentrations of progesterone by employing the microtube culture technique. The changes of transport and macromolecular synthesis systems of the embryos were examined by measuring the amounts of uptake and incorporation of the precursors. The results obtained were as follows: 1. Progesterone stimulated markedly the uptake of amino acids, but rather suppressed their incorporation by embryonic cells. 2. Progesterone suppressed both the uptake and incorporation of nucleotide precursors (uridine and thymidine) by embryonic cells. 3. Progesterone penetrated into the embryonic cell membranes and was taken up by them. The present results seem to indicate that the inhibition of the progesterone on the mammalian embryogenesis in vitro may not be directly related to the membrane transport system. They seem to imply that progesterone would penetrate into the embryonic cells and may directly block the biosynethetic pathways of macromolecules, and so lead to the inhibition of the embryogenesis in vitro.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|